生化学と分析生化学

概要

Study of Role of Surfactant Protein-D, Malondialdehyde, Protein Carbonyl and its Correlation with Airflow Obstruction (FEV1% Predicted) in Patients with Smoker Chronic Obstructive Pulmonary Disease (COPD)

Rupali S Pawar and Subhodini A Abhang

Background: Smokers lungs are exposed to rich amounts of oxidants. Oxidative stress and inflammation are hallmarks of chronic obstructive pulmonary disease. SP-D is lung specific protein play important role in the lungs including regulation of surfactant homeostasis in the alveoli and modulation of host defense system in the lung. The aim of this study was to examine role of surfactant protein-D, malondiladehyde and protein carbonyl in smoker COPD patients and to see whether there is any correlation between pulmonary function test with MDA, PC and SP-D in COPD patients. Materials and methods: We measured serum SP-D, MDA and PC in 30 smoker COPD patients, 30 non-smoker COPD patients and in 30 healthy controls by ELISA and spectrophotometric methods respectively. Results: Serum levels of SP-D, MDA and PC were significantly higher in smoker COPD patients than non-smoker COPD patients. SP-D, MDA and PC were significantly increased in smoker COPD patients as compared to healthy controls. We found inverse correlation between FEV1% predicted with SP-D, MDA and PC in COPD patients. MDA and PC were directly correlated with SP-D in COPD patients. Conclusion: From these findings we conclude that deleterious effect tobacco smoke causes lipid and protein oxidation and lung tissue damage. Lung tissue injury causes release of SP-D in the blood stream. This is directly related with extent of injury and pulmonary function in COPD patients.