生化学と分析生化学

概要

Escherichia coli Generate Oxidative Stress and Enhance Lipid Peroxidation in the Kidney of the Rat

Amiy Dutt Chaturvedi, Nagarajan K, Dharm Pal and Awanish Kumar

The urinary tract is generally infected by Escherichia coli bacteria. They commonly originate in the patient's own bowel, and infection occurs mostly via the ascending route. In this study, we have observed the influence of E. coli on oxidative stress generation and lipid peroxidation in the kidney of the rat. E. coli were taken from the soil, urine, buffalo intestine and goat intestine for this study. Rats were infected with isolated E. coli from different sources and Lipid peroxidation, glutathione assay was done to achieve the goal of this study. Percentage survival data showed that the E. coli isolated from urine had more lethargy phenomena because their survival present was 66.66% and the mortality rate was higher in this group. Although E. coli isolated from Goat intestine has also shown the same mortality, but the E. coli isolated from urine sample shown this mortality from the second day whereas the E. coli isolated from goat intestine from the third day. By and large data indicated that the E. coli isolated from urine sample generated high oxidative stress and damage rat kidney because the kidney is an organ frequently exposed to oxidative stress. Overall, free radical generated due to E. coli infection further enhances lipid peroxidation in the rat which is harmful to the physiology because it may cause a renal disorder in the rat.