生化学と分析生化学

概要

Cellular Toxicity Mechanisms of TiCN-Cp-Ti Screw on Tooth Gum Cells in Rat

Parvaneh Naserzadeh1, Abbas Razmi2, Ruhi Yesildal2*, Behnaz Ashtari1,3,4*

Cellular toxicity mechanism of TiCN film deposited on Cp-Ti substrate by Cathodic Arc Physical Vapor Deposition (CAPVD) method has yet to be clarified. The present study is aimed at finding out the possible effects of synthesized TiCN on the isolated tooth gum cells and realizing its cytotoxicity mechanism under experimental conditions compared to that of Au screws (standard in medicine). Results of the XRD analysis showed that TiCN film was formed in coating. The SEM image of TiCN film displayed a rough and irregular morphology and fine-grained surface. In addition, some other features such as cell viability, the level of Reactive Oxygen Species (ROS), lipid peroxidation (MDA), Glutathione Count (GSH and GSSG), Adenosine Triphosphate (ATP) were examined. On the other hand, activity of succinate dehydrogenase (complex II), NADH dehydrogenase (complex I), Coenzyme Q-cytochrome c reductase/Cytochrome b (complex III) and cytochrome c oxidase (complex IV) were also determined. Finally, it was also determined that level of Alkaline Phosphatase (ALP), Aspartate Amino Transferase (AST), Alanine Aminotransferase (ALP), Urea, Creatinine Clearance (CR) in rat (tooth gum cells) could also change. The results of the study revealed that TiCN coating did not incur an extremely change on cellular toxicity biomarker compared to Au standard screw in animal study. Our study provides the first evidence that TiCN coating is a biocompatible material in the cell. This paper suggests that the use of the TiCN in human dental applications need further examinations in a wide range of cellular-death signaling.