アグロテクノロジー

概要

Wide Hybridization and Embryo-Rescue for Crop Improvement in Solanum

Herbert P. Kharkongar, V.K. Khanna*, W. Tyagi, M. Rai, and N.T. Meetei

Tomato is known in the literature as Solanum lycopersicum, as well as Lycopersicon esculentum. In north eastern region of India, cultivation of tomato in rice fallow is becoming popular and may be helpful in increasing production of vegetables, which will not only increase per capita availability of vegetables, but also improve the economic condition of the farmers through employment generation. Tomato is highly prone to biotic stresses, especially diseases, insects and nematodes. Genes are available in different wild species, but it has not been easy to transfer these genes in cultivated species due to problems in crossability. Solanum lycopersicum was crossed with S. peruvianum and Solanum pimpinellofolium. 25 days after pollination was found to be the optimum time for rescuing the embryos.
Murashige and Skoog’s (MS) medium supplemented with 1 mg/l GA3, 0.1 mg/l NAA and 0.5 mg/l BAP was found to be the most effective for germination of the immature putative hybrid embryos. The confirmation of hybridity of the embryo rescued plants from the interspecific crosses of both S. lycopersicum var. MT-3 and S. lycopersicum var. Kashi Amrit with S. peruvianum (WIR-3957) was done using RAPD markers.