生物学と医学

概要

Use of restriction fragment length polymorphism to characterize methicillin-resistant Staphylococcus aureus in dairy products

M Salehi, V Razavilar, H Mirzaei, A Javadi, SM Banan Khojasteh

Staphylococcus aureus is one of the frequent causes of animal and human infections. In the present study, methicillinresistant S. aureus (MRSA) was investigated in dairy products in Iran. From 116 coagulase-positive S. aureus isolates, 7 samples had MRSA gene. Amplification of MRSA gene produced 1 polymerase chain reaction product with the size of 530 bp. By analyzing the restriction fragment length polymorphism (RFLP) of MRSA gene with Msa1 restriction enzyme, there was no restriction pattern. The results showed that there was no genetic diversity in MRSA gene in S. aureus isolates from dairy products of different areas in East-Azerbaijan Province, Iran.