生物学と医学

概要

The Determination of Oxidized Proteins and Albumin in Plasma of Chronic Kidney Disease Patients

Larissa Evgenyevna Muravlyova, Vilen Borisovich Molotov-Luchanskiy, Ryszhan Yemelyevna Bakirova, Yevgenia Aleksandrovna Kolesnikova, Assel Serikovna Nurgaliyeva, Dmitriy Anatolyevich Klyuyev and Lyudmila Andreevna Demidchik

Background: The study of oxidized proteins in blood of chronic kidney disease patients has been made without taking an initial clinical form of disease into account. The purpose of the research is to study oxidized proteins and albumin in blood plasma of patients with chronic glomerulonephritis and chronic pyelonephritis as an initial clinical form of chronic kidney disease. Methods: Blood plasma taken from 132 patients with various stages of chronic kidney disease and degree of chronic renal failure has been used for investigation. Blood of 32 healthy donors has been used for control testing. In blood plasma, modified proteins (protein reactive carbonyl products, advanced oxidative protein products), and albumin have been estimated. Results: In blood plasma of patients with chronic glomerulonephritis, as an initial clinical form of the chronic kidney disease, the significant increase in all types of oxidized proteins and significant decrease in albumin have been observed, comparing to the control samples. In blood plasma of patients with chronic pyelonephritis, as an initial clinical form of the chronic kidney disease, the significant increase in protein reactive carbonyl products has been observed comparing to the control samples only. Conclusions: the obtained results may unravel new targets for pharmacological intervention at an early stage of chronic kidney disease based on the initial clinical form of the disease