発がんと突然変異誘発のジャーナル

概要

Research of Bovine Papillomaviruses Types 1 and 2 in the Genital Tract of Cows

Talita de Paula Silva Moura, Liria Hiromi Okuda, Marta Elizabeth Scarelli Vicente, Edviges Maristela Pituco, Claudia Del Fava

Papillomaviruses are oncogenic viruses, causing papillomas and fibropapillomas. Bovine papillomavirus-1 (BPV-1) DNA has been found in cow uterine flushings, and BPV-2 in cow ovarian and uterine tissues, uterine flushing, oocytes, and cumulus cells. So far, has been proven BVP presence in the uterus of cattle through molecular techniques, but the pathogen association with uterine lesions is yet not clear. The presence of BPV-1 and 2 was investigated in the genital tract of 80 cows. Eighty dairy cows between three and five years of age were slaughtered and sampled for laboratory analysis. Uterine cervix cytology samples for Papanicolaou testing were collected with a swab smeared in glass slides and fixated with spray. Reproductive organ fragments (Ovaries, uterine tubes, uterine horns, uterine body, and cervix) were collected for histopathology and fixed in 10% buffered formalin. Tissue fragments were collected in a sterile/stereo universal collecting cup, and whole blood was collected in a vacuum tube with EDTA, both frozen at -20˚C for further nested-PCR. Different techniques were used: macroscopy, histopathology (hematoxylin and eosin stain -HE), cytologic atypia of the uterine cervix (Papanicolaou), nested-PCR for the L1 gene of BPV viral capsid using the primers FAP59/FAP 64, and Delta Epsilon F/Delta Epsilon R. Both metritis and endometritis were macroscopically found in 5.0% of cows. Cytology showed cell atypia in 25.0% of cows: karyomegaly, binucleation, and multinucleation, while histopathology confirmed nonspecific endometritis in 25.0% of cows. BPV was not detected by nested-PCR.