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Quantitative Estimation of Dapagliflozin in Blood Plasma by Using UV Spectroscopy
Bhagwat J Bodade, Dhiraj A Kanade and Sandip S Chaudhari
An accurate, precise and reproducible quantitative estimation of dapagliflozin in blood plasma method were developed. Spectrophotometric estimation was done by absorption method followed by using sample of blood plasma of patient who are given dapagliflozin tablet. In this method λmax for DAPA was selected at 224 nm at concentration range of 20-100 μg/ml for DAPA in UV spectrophotometric method (the value of r2=0.994). The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Therefore this method is useful for quantitative estimation of drug and amount of drug absorb in blood plasma.