微生物および生化学技術ジャーナル

概要

Production of Bacterial Cellulose from Gluconacetobacter persimmonis GH-2 using Dual and Cheaper Carbon Sources

Basavaraj Hungund, Shruti Prabhu, Chetana Shetty, Srilekha Acharya, Veena Prabhu and Gupta SG

Bacterial cellulose is an exopolysaccharide produced by various species of bacteria such as in the genera Gluconacetobacter, Agrobacterium, Achromobacter, Azotobacter, Rhizobium, Sarcina, Salmonella, Enterobacter, etc. In recent years, bacterial cellulose has been focused for the development of acoustic diaphragms, specialty membranes, biomedical wound care products, scaffold for tissue engineering, etc. In this study, effective culture method to produce bacterial cellulose from cheaper and dual carbon sources by Gluconacetobacter persimmonis was examined. Various fruit juices including pineapple, pomegranate, muskmelon, water melon, tomato, orange, and also molasses, starch hydrolyzate, sugarcane juice, coconut water, coconut milk were used as alternative carbon sources for bacterial cellulose production. Out of which muskmelon gave a highest cellulose yield of 8.08 g/L. Also glucose, fructose, sucrose, maltose, lactose, mannitol, inositol and glycerol were used in combination of two (1:1) as dual carbon sources. Out of these dual carbon sources, the combination of fructose and sucrose (1:1) gave the highest cellulose yield of 8.79 g/L. In this study, an attempt was made to reduce the cost of production medium for cellulose by using natural cheaper carbon sources.