インデックス付き
  • 学術雑誌データベース
  • Genamics JournalSeek
  • アカデミックキー
  • ジャーナル目次
  • 中国国家知識基盤 (CNKI)
  • シマゴ
  • Global Online Research in Agriculture (AGORA) へのアクセス
  • 電子ジャーナルライブラリ
  • レフシーク
  • 研究ジャーナル索引作成ディレクトリ (DRJI)
  • ハムダード大学
  • エブスコ アリゾナ州
  • OCLC-WorldCat
  • SWBオンラインカタログ
  • 仮想生物学図書館 (vifabio)
  • パブロン
  • ミアル
  • 大学補助金委員会
  • ジュネーブ医学教育研究財団
  • ユーロパブ
  • Google スカラー
このページをシェアする
ジャーナルチラシ
Flyer image

概要

Process Optimization of L-Glutaminase Production; a Tumour Inhibitor from Marine Endophytic Isolate Aspergillus sp. ALAA-2000

Mervat Morsy Abbas Ahmed, Taher M Taha, Nageh F Abo-Dahab and Fareed SM Hassan

L-Glutaminases have received significant attention recently owing to their potential applications. All endophytic fungi recovered from the marine soft sponge Aplysina fistularis were able to produce L-glutaminase. During screening program, Aspergillus sp. ALAA-2000 showed the highest L-glutaminase production levels. The production of L-glutaminase by Aspergillus sp. ALAA-2000 was evaluated under different fermentation modes and parameters. The L-glutaminase synthesis was increased their yield after the optimization of fermentation parameters. The hot water 40°C was the best leaching agent extracted of soy bean for L-glutaminase production (21.89 U/ml) under solid state fermentation (SSF). The highest L-glutaminase activity (91.92 U/ml) was achieved after two days incubation period under submerged fermentation (SmF). L-glutamine, dextrose, cysteine and Magnesium chloride supported the highest L-glutaminase production by Aspergillus sp. ALAA-2000 under SmF at pH 4 and 27°C. Single peak of L-glutaminase was obtained from the culture supernatant of Aspergillus sp. ALAA-2000 through ammonium sulfate precipitation and DEAE-cellulose column chromatographyrefer to the mono meric nature of L-glutaminase enzyme. The parameters of purified L-glutaminase were optimized as follow: pH 10, stable at 40°C to 50°C, reaction time 30 min and substrate concentration 4.38 mg/ml. Whereas the maximum activator cation is Na+ and different EDTA concentrations have no effect on L-glutaminase activity which means that L-glutaminase enzymes was represent as a non-metallic enzyme.

免責事項: この要約は人工知能ツールを使用して翻訳されており、まだレビューまたは確認されていません