石油および環境バイオテクノロジージャーナル

概要

Potential of New Microbial Isolates for Biosurfactant Production using Combinations of Distillery Waste with other Industrial Wastes

Kirti V Dubey, Pravin N Charde, Sudhir U Meshram, Santosh K Yadav, Sanjeev Singh and Asha A Juwarkar

In the present study, combinations of Distillery Waste (DW) with other industrial wastes viz. curd Whey Waste (WW), Fruit Processing Waste (FPW) and Sugar Industry Effluent (SIE) were evaluated to replace the use of water that was reported earlier for biosurfactant production from 1: 3 diluted distillery waste by using four new bacterial cultures BS-A, BS-J, BS-K and BS-P, isolated from soil collected from a distillery unit. These isolates have the potential to produce biosurfactant from these individual wastes and in their combinations. Highest biomass and biosurfactant yields with higher reduction in the Chemical Oxygen Demand (COD), total sugars, nitrogen and phosphate levels were obtained in 1:1:1 proportion of DW+WW+FPW followed by DW+WW+ SIE and individual wastes. The combinations of wastes improved the yields of biosurfactants by 18-41% and reduced COD of the combined wastes by 76-84.2%. Total sugars, nitrogen and phosphate levels reduced in the range of 79-86%, 58-71% and 45-59%, respectively. Among the four microbial isolates tested, BS-J and BS-P were the efficient biosurfactant producers and were identified as Kocuria turfanesis and Pseudomonas aeruginosa based on the 16S rDNA sequence and phylogenetic analyses. Benefits derived by using combined distillery waste with other wastes are improved production of biosurfactant as resource and saving precious water and the costly nutrients with concomitant reduction in pollution load of the wastes.