水産養殖研究開発ジャーナル

概要

Nucleotides and Effect Over Starving Condition on Fish SHK-1 Cells Model

Pamela Olivares, Rodrigo Sanchez, Erico Carmona, Allisson Astuya, Hector Herrera and Jorge Parodi

Additives play an important role in nutrition for boosting the physiological processes of productive species, and starvation has become a new problem for the industry. Only in recent years have studies began to analyze the effects of additives on fish cells. We observed the effects of nucleotides, which is a compound highly utilized in aquaculture. The SHK-1 cell line was derived from the head and kidney of Atlantic salmon as a biological model. Samples were exposed to nucleotides at incremental times and concentrations to determine the effects on cell viability by evaluating the number of cells. The cells were also exposed to a starving condition and recovery when nucleotides were used. Our data indicate that nucleotides are not sufficient to stimulate cellular proliferation. Cultures were exposed to a basal medium without serum in the presence of or absence of nucleotides. We observed that the starving effects are reduced when the basal medium is supplemented with nucleotides. Our results indicate that it is important to evaluate additive effects at a cellular level and that nucleotides have nutritional effects when the cells are in the cellular starving condition. Our study helps to generate more rational applications of additives in the industry and presents new challenges.