ナノメディシンとナノテクノロジーのジャーナル

概要

NIR-Light-Driven Generation of Reactive Oxygen Species Using Ru(II)- Decorated Lipid-Encapsulated Upconverting Nanoparticles

Aruna Reddy

The natural use of ruthenium anticancer prodrugs for photodynamic treatment (PDT) and photoactivated chemotherapy (PACT) is limited by the need to utilize inadequately infiltrating high-energy photons for their enactment, i.e., regularly blue or green light. Upconverting nanoparticles (UCNPs), which produce high-energy light under close infrared (NIR) excitation, may explain this issue, given that the coupling between the UCNP surface and the Ru prodrug is improved to deliver stable nanoconjugates with effective energy move from the UCNP to the ruthenium complex. In this, we report on the blend and photochemistry of the two basically related ruthenium(II) polypyridyl buildings [Ru(bpy)2(5)](PF6)2 ([1](PF6)2) and [Ru(bpy)2(6)](PF6)2 ([2](PF6)2), where bpy = 2,2-bipyridine, 5 is 5,6-bis(dodecyloxy)- 2,9-dimethyl-1,10-phenanthroline, and 6 is 5,6-bis(dodecyloxy)- 1,10-phenanthroline. [1](PF6)2 is photolabile because of the steric strain initiated by ligand 5, however the illumination of [1](PF6)2 in arrangement prompts the nonselective and moderate photosubstitution of one of its three ligands, making it a helpless PACT compound. Then again, [2](PF6)2 is a productive and photostable PDT photosensitizer. The water-dispersible, contrarily charged nanoconjugate UCNP@lipid/[2] was set up by the epitome of 44 nm measurement NaYF4:Yb3+,Tm3+ UCNPs in a combination of 1,2-dioleoyl-sn-glycero-3-phosphate and 1,2-dioleoyl-sn-glycero-3-phosphocholine phospholipids, cholesterol, and the amphiphilic complex [2](PF6)2. A nonradiative energy move productivity of 12% between the Tm3+ particles in the UCNP and the Ru2+ acceptor [2]2+ was discovered utilizing time-settled outflow spectroscopy. Under illumination with NIR light (969 nm), UCNP@lipid/[2] was found to create responsive oxygen species (ROS), as decided by the oxidation of the vague ROS test 2′,7′-dichlorodihydrofluorescein (DCFH2–). Assurance of the sort of ROS created was blocked by the negative surface charge of the nanoconjugate, which brought about the electrostatic shock of the more explicit yet additionally adversely charged 1O2 test tetrasodium 9,10-anthracenediyl-bis(methylene)dimalonate (Na4(ADMBMA)).