微生物および生化学技術ジャーナル

概要

New Trends in Protein Nanocrystallography Based On LB Nanotemplate, Cell Free Expression, SNAP APA and Montecarlo: A Review

Claudio Nicolini and Eugenia Pechkova

A review of the emerging trends and perspectives of Protein Nanocrystallography is here presented at the intersection of advances in nanotechnology (Langmuir-Blodgett and Anodic Porous Allumina), proteomics (microarray, cell free expression and SNAP) and synchrotron radiation (third generation sources trillion times more brilliant requiring quite smaller crystals and Montecarlo simulation). It should be noted that nanocrystallography here does not refer to crystals of nanometer size or to nanodrop crystallization technology, but to the significant applications for medicine emerging in our labs at the interface of Langmuir-Blodgett engineering, organic chemistry, molecular dynamics and label-free Protein Arrays, utilizing bacterial hell's gate globin, octapus rodhopsin, bovine cytochrome, human kinase, laccase and many other proteins.