微生物および生化学技術ジャーナル

概要

Modeling and Optimization of 1-Kestose Production by Schedonorus arundinaceus 1-SST

Duniesky Martínez, Roberto J. Cabrera, Iván Rodríguez, Carmen Menéndez, Alina Sobrino, Lázaro Hernández, Enrique R. Pérez*

In current commercial mixtures of inulin-type fructooligosaccharides (FOS) synthesized from sucrose by fungal fructosyltransferases, 1-kestose has superior bifidus-stimulating effect than nystose and frutosyl-nystose. In this study, a recombinant sucrose:sucrose 1-fructosyltransferase (1-SST, EC 2.4.1.99) from the plant Schedonorus arundinaceus (Sa) was the enzyme of choice to maximize the batch production of 1-kestose in a jacketed agitated vessel. Mathematical models were developed to predict the optimal conditions for the sucrose conversion reaction and the subsequent enzyme inactivation to prevent 1-kestose hydrolysis. After programed heat inactivation of Sa1-SSTrec in optimized batch experiments with different enzyme and sucrose concentrations, 1-kestose represented more than 90% of total FOS content (53%-58%, w/w) in the reaction mixture. The mathematical models described herein are suitable tools for the cost-effective production of 1-kestose in scaled batch reactions.