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Method Development and Validation for Simultaneous Estimation of Dexlansoprazole and Meloxicam by Rp-Hplc
Jalli Sriharsha, Srinivasa Murthy M, Bharat Kumar D, Sravan K, Shiva kumar P, Shirisha A and Pranusha K
To develop a method for simultaneous estimation of Dexlansoprazole and Meloxicam by RP-HPLC and validate the developed method according to ICH guidelines. The column used for method development of Dexlansoprazole and Meloxicam was Hypersil-BDS, C18, 250*4.6 mm, 5μ. Methanol and Acetonitrile were used in the ration of 60:40 for the method development. The developed method was validated for various parameters such as specificity, linearity, range, accuracy, precision, system suitability, robustness, ruggedness etc. The method was developed and optimized method was chosen and validated and the results were tabulated according to ICH guidelines. The result obtained in this study demonstrated that the HPLC method described is specific, accurate, precise, linear, ruggedness, robustness and stability indicating for the simultaneous determination of Dexlansoprazole and Meloxicam.