細菌学および寄生虫学ジャーナル

概要

Involvement of Pyoverdin, Proteolytic and Lipolytic Enzymes in Pseudomonas aeruginosa Biofilm Formation

Yao Paul Attien, Comoé Koffi Donatien Benié2, Haziz Orou Sina, Wako-Tianwa Alice Tuo, Arthur Zebre, Clément Kouassi Kouassi, Ibrahim Konaté, Lamine Baba Moussa, Adjehi Dadie, Mireille Dosso

The pathogenicity of P. aeruginosa, which is associated to acute and chronic infections, is related to its ability to form biofilms, and produce spoilage determinants such as pyoverdin, proteases, lipases, and toxins. The purpose of this research was to determine the involvement of pyoverdin, proteolytic and lipolytic enzymes in biofilm formation. A total of 33 strains of P. aeruginosa of animal (11), environmental (11) and clinical (11) origin were identified by PCR followed by sequencing. The microplate method was used to quantify biofilm formation. The pyoverdin on medium was determined by spectrofluorometry. The production of protease and lipase was sought respectively on agar with milk and with Rhodamine B on solid Luria-Bertani medium. In decreasing order of importance, the median value of biofilm formed was 1.5 (environmental strains); 1.3 (clinical strains) and 1.2 (animal strains). Biofilm categories were categorized into strong (37% to 48%), moderate (28% to 45%), and weak (6% to 14%) producers. The average pyoverdin production was 1304.4 nm, 1325.1 nm and 999.1 nm respectively in animal strains, environmental clinics. The production of protease ranged from 85.0% to 100% while that of lipase ranged from 70.0% to 90.6%. Control of certain virulence factors may reduce the pathogenicity of P. aeruginosa.