水産養殖研究開発ジャーナル

概要

Identification of Protease Synthesizing Micrococcus Yunnanensis from the Gut of Mud Crab Scylla Serrata

Anu Mathew*

In the present study screening of bacteria from the gut of mud crab Scylla serrata collected from Karwar coast (N-13°,05.722‘; E- 079°, 48.658‘), Karnataka has been resulted in the isolation of a yellow pigmented, protease producing gram positive bacterial strain AN-06. It was able to utilize casein as substrate for growth and showed zones of clearance around the colony in Skim milk agar medium, and hence was selected for molecular characterization by 16S characteristics and 16S rRNA sequence analysis (99% similarity) (Genbank accession number KU937307). observed at temperature, 28°C; pH, 8.0 and NaCl, 15%. There is only very little information on gut microbes from marine crab and their functional diversity. It is the first time report on the occurrence of protease producing digestion in marine crabs. However, detailed studies are required to know the source and exact role of this bacterium Keywords: Scylla serrata; Micrococcus yunnanensis; Protease; Gut bacteria INTRODUCTION The digestive tract of both marine as well as freshwater fishes host regular microflora, which are autochthonous and allochthonous [1]. Information on the interaction between the gut microbiota and intestinal immunity in fish and crustaceans is available is remarkably less. The connections between gut flora and host are one of the most important factors that influence aquatic animal health [2]. The intestinal flora has been reported as vital to the development [3].Immunity and disease resistance of gut [4,5]. Gastrointestinal bacteria also have significant role in nutrients digestion and provide the host with physiologically active materials, like enzymes, amino acids and vitamins Symbiotic bacteria in an animal’s digestive tract often produce enzymes that would complement for digestion of foods as well as synthesize compounds that are assimilated by the host [6]. Harris suggested that gut bacteria may contribute significantly to nutrient gain by aquatic hosts [7,8]. Giant prawn [9] prawns upogenbia africana and Callinessa kraussi sea shown to maintain a permanent and consistent microbiota in the gut, which is significantly different from that of the surroundings. In many studies there is no indication to suggest the bacteria isolated from the gut are anything other than ingested, transient bacteria. Nagasawa and Nemoto reported that bacteria may be an important food source for some marine invertebrates . The most commonly isolated bacterial genera are in the digestive process, growth and disease resistance of the host although few reports concerning microbial enzyme production in the gastrointestinal tract of fish are available [11] information on the distribution of these enzyme-producing endosymbionts in different regions of the gut are scarce [12]. Enzymes are considered as a vital resource utilized by the food, chemical and allied industries to produce a wide range of biotechnology products and have already been recognized as valuable catalysts for various organic transformations and Journal of Aquaculture Research and Development Research Article Corresponding author: Mathew Anu, Department of Pharmaceutical Chemistry, Nirmala College of Pharmacy Muvattupuzha, Kerala, India, Email: anuchalilm@gmail.com Received date: April 01, 2021; Accepted date: April 14, 2021; Published date: April 21, 2021 Citation: Mathew A (2021) Identification of Protease Synthesizing Micrococcus Yunnanensis from the Gut of Mud Crab Scylla Serrata. J Aquac Res Dev.12:589. Copyright: © 2021 Mathew A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. J Aqua Res Dev , Vol.12 Iss.4 No:1000589 1 rRNA sequencing. The strain AN-06 was identified as Micrococcus yunnanensis based on the phenotypic Micrococcus yunnanensis is gram-positive, aerobic, non-endospore-forming, non- motile cocci. Optimal growth was Micrococcus yunnanensis from the gut of a healthy marine crab. The role of these bacteria may be for protein In the present study screening of bacteria from the gut of mud crab Scylla serrata collected from Karwar coast (N-13°, in Scylla serrata gut