水産養殖研究開発ジャーナル

概要

Identification and Typing of Aeromonas Hydrophila through 16S rDNA-PCR Fingerprinting

Agniswar Sarkar *,Mousumi Saha ,Pranab Roy

16S rRNA is a conserved biomolecule within a cell. Sequencing data was analyzed and examined by sequencing the corresponding 16s rDNA regions of isolates from different sources. Specific culture media and different biochemical tests primarily confirmed isolates as Aeromonas hydrophila; those were designed and tested following PCR assays and identified by 16S rDNA gene sequence analysis. To automate the method few online and offline computational tools have been used. The method has proven useful for identification of Aeromonas species. Our results emphasize the need to take into account the intragenomic diversity of the 16S rRNA gene. Different software and programme used in this study are freely available online in different website.