ファーマシューティカ アナリティカ アクタ

概要

HPTLC and RP-HPTLC Method Development and Validation for the Estimation of Felodipine in Bulk and Pharmaceutical Formulation

Jain PS, Ansari NA and Surana SJ

The aim of this work is to establish developed and validated method for the pharmaceutical analysis of Felodipine in bulk and pharmaceutical formulation by High Performance Thin Layer Chromatography HPTLC (NP) and Reverse Phase-High Performance Thin Layer Chromatography RP-HPTLC (RP). Chromatographic separation was performed on Pre-coated aluminum plates with 250 μm layer of Silica gel 60 F₂₅₄ and Silica gel 60 RP-18 TLC F₂₅₄S using Toluene: Methanol (8:2 v/v) and acetonitrile: water: glacial acetic acid (8:2:1 v/v/v) as a mobile phase, respectively. Scanning was carried out densitometrically at 237 nm. The R_f value of Felodipine in NP and RP were 0.40 and 0.53 and the reliability of the method was assessed by the evaluation of linearity which was found to be 300-1800 and 500-3000 ng/band with the r² =0.998 correlation coefficient along with the accuracy of the method in terms of % recovery was found to be from 98-101 ± 1.04 % and 99-100 ± 0.47 % and the limit of detection and quantification were 11.51, 34.90 and 29.90, 90.61, respectively. The method can be used for routine analysis of Felodipine in bulk and pharmaceutical formulation.