ナノメディシンとナノテクノロジーのジャーナル

概要

Gold Nanosensitisers for Multimodal Optical Diagnostic Imaging and Therapy of Cancer

Vijay Raghavan, Jennifer M Connolly, Hai Ming Fan, Peter Dockery, Antony Wheatley, Ivan Keogh and Malini Olivo

Multi-modal nanoprobes have attracted much attention because of their superior chemical and physical properties that have great potential for early diagnosis and treatment of cancer. We report herein a novel stepwise fabrication of a gold nanoparticle-Raman reporter-photosensitiser conjugate called a Gold Nanosensitiser (NS). The efficacy of gold NS as multimodal nanoprobes for surface enhanced Raman spectroscopy (SERS) imaging and photodynamic (PDT) and photothermal treatment (PTT) of cancer were examined. NS were constructed using a multilayer technique in which a Raman reporter, DTTCI (3,3’-Diethylthiatricarbocyanine iodide) and a photosensitiser, hypericin, were coated onto gold nanostars. By adjusting the layer spacing, these coatings endowed NS with both enhanced SERS and fluorescence bioimaging capabilities. Excitation of the NS in solution at an appropriate wavelength permitted examination of the photodynamic therapy (PDT) capability in terms of singlet oxygen generation. As the nanostar is multibranched and comprises multiple ‘hot spots’ capable of enhanced electromagnetic radiation, along with SERS it also exhibits photothermal therapy (PTT) capability when excited at its resonant wavelength of 675 nm. The NS was also characterised to examine the retention of physio-chemical and optical properties of its components after fabrication. The development of such a multimodal nanoplatform in the current work will offer a useful tool for noninvasive, high-accuracy, single-node diagnosis and therapy of cancer.