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Genetic and Biochemical Characterization of Some Egyptian Rhizobia Isolates Nodulating Faba Bean
Abdel Fattah M Elzanaty, Omar A Hewedy, Khalid S Abdel-lateif, Hisham H Nagaty and Mohamed I Abd Elbary
Ten Rhizobial isolates were isolated from root nodules of Faba bean plants (Viciae faba L.) grown in different soil types and representing different geographic locations in Egypt. The isolates were characterized biochemically for their production of eachIAA and catalase. The tested isolates differed in their IAA production. The maximum production of IAA production was recorded for RLZ isolate of Zefta with value of 4.56 μg/ml, while the lowest production was recorded for RLK isolate of Kaha with 2.04 μg/ml. Moreover, all isolates were positive in their production of Catalase enzyme except RLZ isolate. The isolates were evaluated for their plasmid content and profiles produced after cutting by two restriction endonucleases (EcoRI and MSPI). SDS-PAGE analysis was used to distinguish between the different isolates based on their banding protein patterns.