法医学バイオメカニクスジャーナル

概要

DNA Profiling of Transgenes in Genetically Modified Plants

Bittsánszky A, Gabor G, Gullner G and Komíves T

In silico sequence diversities of four orthologous plant gsh1 genes and their anino acid translates of GSH1 proteins (Glutathione Synthase) were compared to the non-orthologous prokaryotic gshI/GSHI gene/protein of E. coli (NCBI # X03954). Primer pair was designed and transgene detection was carried out in two types of gshI-transgenic poplar clones (Populus x canescens) of ggs11 (cyt-ECS) and lgl6 (chl-ECS). Usefulness of genetic modification technologies (GMO) is indicated.