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Determination of the Endocrine Disrupter Bisphenoi-A in the Blood of Uremia Patients Treated by Dialysis
H. Shintani and F. Hayashi
Automated solid-phase extraction (SPE) then high-performance liquid chromatography have been studied for determination of the endocrine disrupter bisphenoi-A (BPA) in blood. Electro chemical detection was used for selective and sensitive detection of BPA. Determination of BPA in the blood of uremia patients treated by dialysis has not yet been reported. Acidified blood and acidified SPE eluent were used to suppress the ionization of BPA and thus retain the compound on a C-18 column. Because artificial dialysis can be performed for periods longer than 40 years, for reasons of safety the amount of BPA migrating from the artificial dialyzer into the blood of a uremia patient was compared for different sterilization methods and for different artificial dialyzers manufactured from different materials.