歯科

概要

Assessment of Bone Neoformation in Dental Alveolus with Platelet-Rich Plasma (PRP) in Rabbits (Oryctolagus cuniculus).

Panhoca VH, Bagnato VS and Tamae PE

Objective: The purpose of this work was to assess the bone neoformation of a dental alveolus in PRP-grafted rabbits by means of a toluidine blue-stained experimental model using conventional light microscopy.

Material and Method: It was used thirty male rabbits of the New Zeland breed, divided into 5 control groups and 5 experimental groups. Each group was divided into subgroups to assess the postsurgery period after three, four, and eight weeks. Every rabbit was submitted to exodontia of the Right Lower Incisor (RLI) and its antagonist. Only the experimental group received PRP inside the RLI alveolus. The bone calcein-marker was ministered to every animal in the first and penultimate postsurgery week before sacrificing.

Results: Upon the analysis of the toluidine blue-stained blades, a cellular maturation was observed with intense osteogenic activity in the experimental group. When analyzing the fluorescence microscopy, it was verified that the bone neoformation presented a constant evolution in the 3, 4, and 8 week period in the control group. As to the experimental group, a quite significant peak was observed in the 4 week period compared to the control group p<0.05.

Conclusion: It was verified that the application of PRP propitiated a real acceleration of the bone neoformation.