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Antiradical Activity and Total Phenolics of Algerian Honeys and Antibacterial Effect against Gram-Negative Bacteria
Moussa Ahmed, Noureddine Djebli, Saad Aissat, Baghdad Khiati, Meral Ünal and Salima Bacha
Six Algerian honeys from different floral origins, were examined for potential antibacterial and antiradical activity. The Folin-Ciocalteu assay was used to measure total phenol content (TPC) and the 2,2-diphenyl-picrylhydrazyl (DPPH) assay was used to determine the scavenging activity of the honey samples. An agar well diffusion assay and spectrophotometric method were used to assess antibacterial activity against two Gram negative strains (Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC 50071).Total phenolic content varied from 63.93 to 95.36 mg/100 g honey as gallic acid equivalent. The DPPH radical scavenging assay was found for an average (30.14 % ± 9.28). The honey samples were found to inhibit all of the tested bacteria. Correlation existed between phenolic content and antiradical activity. Thus Algerian honeys, being a rich source of natural antioxidants, may be used in the prevention of various free radicals related diseases.