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Analysis of Escherichia Coli Inducing Salpingitis-Peritonitis in Layer Geese by Two-Dimensional Electrophoresis and MALDI-TOF/TOF
Jin Wen-Jie, Zhang Yong-Pan, Qian Wen-Zheng, Zhang Di, Shao Hong-Xia, Qian Kun and Qin Ai-Jian
Geese salpingitis-peritonitis mainly happens in layer geese during egg peak period and leads serious economic losses. This study was conducted in different resource E. coli inducing Geese salpingitis-peritonitis and Geese or chicken fibrinous pericarditis-perihepatitis-airsacculitis respectively. The molecular mechanism underlying pathogenicity of Geese salpingitis-peritonitis E. coli was investigated through analysis of the special protein of this E. coli using proteomics and bioinformatics techniques. Two-dimensional electrophoresis (2-DE) analysis showed a total of 42 protein spots only expressed in Geese salpingitis-peritonitis E. coli. A total of 21 proteins were identified by MALDI TOF/TOF mass spectral analysis. According to gene ontology (GO) and Pathway analysis, special expressed proteins in Geese salpingitis-peritonitis E. coli were mainly involved in the synthesis of macromolecular material, Cellular metabolism and differentiation, Cytoskeleton composition and other relevant Geese salpingitisperitonitis E. coli, 30S RPS6, KASwere possibly associated with its infection.