ファーマシューティカ アナリティカ アクタ

概要

A Simple, Isocratic and Ultra-Fast Liquid Chromatography / Mass Spectrometry Method for the Estimation of Barnidipine in Human Plasma

Napa Delhiraj and Sockalingam Anbazhagan

An Ultra Fast liquid chromatography – mass spectrometry method (UFLC-MS), for detection and quantification of the anti-hypertensive drug, Barnidipine in human plasma was developed. The Detection was performed using Shimadzu LCMS – 2010 EV mass spectrometer with Atmospheric pressure chemical ionization source in negative polarity. Indapamide was used as an internal standard. Selective ion monitoring (SIM) mode was selected with Barnidipine m/z 491 in negative ion mode. Chromatographic separation of analyte and internal standard were carried out using a reverse phase column, Phenomenex C18 (50 x 4.6 mm i.d., 5 μ) at a flow rate of 0.300 mL/min. Mobile Phase is composed of Acetonitrile: 0.05 % Formic acid (60:40) v/v. Extracted by Solid Phase Extraction with a sample volume of 200 μL plasma. The assay of Barnidipine is linear over the range of 50 ng/mL to 1000 ng/mL with a precision of <9.86%. Mean extraction recovery for Barnidipine was more than 61%. Samples are stable at room temperature for 6 hrs, processed samples were stable at least for 28 hrs and also stable at three freeze–thaw cycles. The limits of quantification and detection were achieved upto 10 ng/ml and 5 ng/ml respectively. This method was applied successfully to determine Barnipine in plasma samples.