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A New Stability-Indicating HPLC Method for Simultaneous Determination of Curcumin and Celecoxib at Single Wavelength: an Application to Nanoparticulate Formulation
Dalapathi B Gugulothu and Vandana B Patravale
A simple, precise, isocratic, reverse phase high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of curcuminoids: viz. curcumin(C), desmethoxycurcumin (DMC), bisdesmethoxycurcumin (BDMC) and celecoxib at single wavelength using a Agilent RP C18, 4.6 mm × 150 mm, 5 μm XDB column. The run time was 18 min. The influence of mobile phase composition, injection volume, mobile phase pH, flow rate, temperature, and detector wavelength on resolution was investigated. The method was validated with respect to precision, accuracy, and linearity. The LOD and LOQ were found to be 0.3 and 1 μg/mL, respectively for curcumin and 0.03 and 0.1 μg/mL for respectively for celecoxib. Linearity range was from 1- 20 μg/ mL for curcumin and from 0.1-2 μg/mL for celecoxib. Further, the proposed method was found to be reproducible and convenient for stability-indicating analysis of curcumin–celecoxib pH sensitive nanoparticles.